play sign allow the force directed layout to start computing again. By default the force layout is paused in order to consume less resources, however for instance after filtering the current selection the user may feel the need to further render the graph (separate the plasmids from each other). This button will change the behavior after being clicked, and then if clicked again it will pause the force layout.shift key + left mouse dragging to select plasmid in a rectangular area. Releasing shift key or left mouse click will release the selection area and color the selected plasmids in orange. To disable this mode, click this button again and it will turn grey again and lock area selection.reset nodes button or by clicking in the shift key without selecting anything.shift key without releasing and making several selections with mouse drag and click. Note: Do not release shift key between multiple selections! If you do so, selection is finished.x key, while still pressing shift key and moving the mouse around. While pressing x key and shift key when you move the mouse (without right or left click or otherwise you will drag the entire graph), the selected plasmids (in orange) will move to according to the mouse movement.JSON files are loaded through the import of mapping, mash screen or sequence results.